Two-dimensional cellulose acetate electrophoresis.
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چکیده
منابع مشابه
Rapid cellulose acetate electrophoresis. I. Serum proteins.
A method of electrophoresis utilizing cellulose acetate is described wherein quantitation of serum protein fractions by densitometry is accomplished within 2 hr. of initiation of the procedure. The densitometer was found to have a linear response to amido black dye. Albumin and y-globulin demonstrated equal dye-binding characteristics. Combined with the absence of albumin trailing, this has aff...
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A modification of the method of haemoglobin electrophoresis using cellulose acetate membrane described by Kohn (1969) enables whole blood to be applied direct to the membrane instead of a haemolysate. The method gives results which are indistinguishable from those obtained using a haemolysate. Normal protein stains are not recommended, because of interference from plasma proteins, but the metho...
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The concentrations of serum immunoglobulins (Ig) C, A, and M were correlated with specific features in the $y region of densitometer patterns of serum protein electrophoresis on cellulose acetate membranes; visual analysis of densitometer patterns permitted semiquantitative prediction of values for lgC and lgA. Important immunoglobulin abnormalities, particularly elevations of IgA, were seen in...
متن کاملRapid electrophoresis and quantitation of haemoglobins on cellulose acetate.
A rapid and reproducible electrophoretic method for the separation and quantitation of haemoglobins on cellulose acetate is described. The accuracy of the method and its possible sources of error are discussed. The normal range for haemoglobin A(2) by this method is 1% to 3% of the total haemoglobin concentration. Blood samples from 32 thalassaemic patients showed haemoglobin A(2) values of 3.5...
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In previous papers (Gavrilescu, Jellinek, and McMenemey, 1961, Gavrilescu and McMenemey, 1964) on comparative studies on the protein fractions in the serum and cerebrospinal fluid in multiple sclerosis we have noted increases in the °C2 and f globulins in the serum. Although not specific for this disease these changes are of interest, particularly as multiple sclerosis is considered to be purel...
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ژورنال
عنوان ژورنال: SEIBUTSU BUTSURI KAGAKU
سال: 1990
ISSN: 0031-9082,1349-9785
DOI: 10.2198/sbk.34.335